Open Access Open Access  Restricted Access Subscription or Fee Access

QUANTIFICATION OF DIOSGENIN BY HPLC AND HPTLC TECHNIQUES FROM IN-VITRO PLANT AND IN-VIVO RAISED COSTUS PICTUS.D.DON PLANTS

RADHA DEVI G.M, T. G. UMESH

Abstract


Costus pictus.D.Don, f. Costaceae, Insulin plant, herbaceous perennial medicinal plant, produces Diosgenin, a biologically important compound used in various pharmaceutical products. Diosgenin is reported as active steroidal sapogenin, occurring naturally as anti-oxidant, steroidal saponin compound. It’s used in pharmaceutical industries as a natural source of steroidal hormones, sugar-free (aglycone) belonging to the triterpene group used for the synthesis of cortisone, pregnenolone, progesterone, etc. It’s also experimentally proved that Diosgenin lower’s the cholesterol level in human cells and shows anti-tumor activity. Presently a study has been conducted on the comparative High Performance liquid Chromatography and High Performance Thin Layer Chromatography analysis of Diosgenin from the dried rhizome of in-vitro and in-vivo grown C.pictus. The rhizome extracts of C.pictus widely used as anti-fertility, anti-oxidative, anti-helmintic properties, known to lower blood glucose levels, hypoglycemic and hypolipidemic activities. Nodal explants of C.pictus cultured on Gamborg’s media supplemented with different concentrations of Triacontanol. Best and fast multiplication response was noticed in B5 25µg /l. The isolation, characterization and determination of Diosgenin by HPLC showed presence of 1.97mg/g dry weight in-vitro plant and 15.95 mg/g dry weight in-vivo plants. The quantitative determination of Diosgenin was performed on silica gel 60 F254 HPTLC plates as stationary phase. The linear ascending development on a twin trough glass chamber CAMAG scanner-3 equipped with CATs software used for spectro-densitometric scanning and analysis were carried out at ultra-violet region λ- 254 and 366nm. Comparative study reveled that bio-active compounds occurred in in-vitro raised plants were true-to-type of C.pictus.

Key words: C.pictus, Diosgenin, Rhizome, Micropropagation, isolation, HPLC, HPTLC.

Full Text:

PDF

References


Bassam Abdul Rassol Hassan (2012) Medicinal plants (Importance and uses); Pharmaceutical Analytic, Acta Vol 3. Issue.10.

Edeoga H.O, Okoli B.E (2000) Chromosomes number of Costus Lucanisanus (Costaceae) in Nigeria, Folia Geobotanica 35:315-318.

Wang T.C; Chuang Y.C; Ku Y.H (2007) Qualitative of bioactive compounds in citrus fruits cultivated in Taiwan. Food Chem., 102, 1163-1171.

Nandhakumar etal (2007); Anti-diabetic activity of Methanolic leaf extract of Costus pictus D.Don in alloxan induced Diabetics rats. J. of Health sciences. 53 (6) 655-663.

Jayasri M.A, Gunasekaran.S.Radha.A. Mathew. T.L; Antidiabetic effect of Costus pictus leaves in normal and streptozotocin- induced diabetes rats. Int.J.Diabetes and Metabolism (2008); 16; 117-122.

Gireesh, G.Thomas S.K. Joseph B, Paulose C.S (2009); Antihyperglycemic and insulin secretory activity of Costus pictus leaf extract in streptozotocin induced diabetes rats and in in-vitro pancreatic islets of langerhan culture. J. Ethno Pharmacology. 123; 470-474.

Jayasri M.A, Radha T.L, Mathew. (2009). α –Amylase and α-Glucosidase inhibitory activity of Costus Pictus D.Don in the Management of diabetes. J of Herbal Medicine and Toxicology 3 (1) 91-94.

Arun Kumar A.R, Bastin T.M, Jenifer S, Arumugam M. (2010); Comparative evaluation of extracts of C.igneus (C.pictus) for hypoglycemic and hypolipidemic activity in alloxan diabetes rats. Int J Pharm Tech 2; 182-195.

Pareeek A, Suthar M, Godavarthri A, Goval M, Bansal V. (2010) Negative Regulation of glucose uptake by Costus pictus in L6 myotube cell line. J Pharm Negative. 1: 24-26.

Al-Romaiyan A, Jayasri M.A, Mathew T.L, Hunang G.C, Amiel S.Jones P.M etal; (2010) Costus pictus extracts stimulate Insulin secretion from mouse and human islets of langerhans. Invitro cell physiol. Bio chem. 26; 1051-1058.

Jose.B.Reddy L.J (2010) Analysis of the essential oil of the stems leaves and rhizomes of the medicinal plant Costus pictus form southern India. Int J of Pharmacy Pharm Sci; 2(supple 2); 100-101.

Stabitha T Issac and Alphonse .J.K. (2011) Comparative study of hypoglycemic activity of Costus pictus & Costus igneus in streptozotocin induced diabetic rats. J Pharm. Res. 4: 3628-3629.

Suganya S, Narmadha R. Gopalakrishna V.K. Devaki K. (2012) Hypoglycemic effect of Costus pictus D.Don on alloxan induced type 2 diabetes mellitus in albino rats. Asian Pac J Trop Diseases. 2; 117-`123.

Annadurai et al; (2012) Next generation sequencing and de-nova transcriptome analysis of Costus pictus.D.Don a non model plant with potent anti-diabetic properties. BMC Genomics. 13, 663. 1-15.

Jyotrimoy etal (2012). Estimation of the anti-proliferative potential of Costus pictus on Molt-4 Human Cancer cell lines. Int J of Biopharmaceutical and toxicology Research Vol. 2 Issue 244-250.

Shiny C.T, Kuldeep Singh Yadav, Narayan Prasad Yadav, Suaib Lugman LMS Palni. (2013). Comparative evaluation of Costus pictus D.Don leaf extracts against glucose challenged mice. Annuals of Phytomedicine 2(2); 57-62.

Ranjitha, V.H, Narayanaswamy.M Krishnaswamy A, Sathyanarayana.M.L; Upendra H.A (2013) Effect of Aqueous extracts of Costus pictus & Solanum nigrum leaves on Blood glucose levels and Histo-architecture of Pancreatic islets in alloxan induced Diabetic rats; Journal of Cell and Tissue Research.

Aruna, A Nandhini.S.R, Karthikeyan V. Bose P. Vijayalakshmi. K.Jegadeesh S. (2014) Insulin Plant (Costus pictus) leaves; Pharmacognostical standardization and phytochemical evaluation. American Journal of Pharmacy and Health Research. 2(8) 106-119.

Ferosekhan A. Ramu and S.Ravi kumar. (2016) scientific evaluation of traditionally known Insulin Plant Costus species for the treatment of Diabetes in Human. Int. Journal of Current Research in Biosciences and Plant Biology.3 (6); 87-91.

L.Joji Reddy and Beena Jose (2010) Evaluation of antibacterial activity of the leaf essential oil Costus pictus D.Don from South India. Int.J of Current Pharmaceutical Research Vol 2 Issue 3 68-70.

G. Sulakshana, A Sabitha Rani and B. saidulu. (2013) Evaluation of anti-bacterial activity in Species of Costus .Int J. Current Microbiology App Sci. 2 (10) 26-30.

Yogita Sardessai, Gauri Pai Angle, Arun Joshi, Sonia Carvalho, Maya bhobe ;( 2014) Anti-bacterial activity of Methanolic extract of the rhizome of Costus igneus. J. Pharmaceutical Chemical and Biological sciences. 2(3) 176-185.

Ramya R. Dhamotharan R. (2016) Antibacterial activity and screening of anti-bacterial compounds of Costus pictus D.Don using GC-MS. Int.J of Science and Research (IJSR) vol 5 Issue 6 June 810-814.

Nadumane V.K, Rajashekar S, Narayana P, Adhinarayana S, Vijayan S, Prakash S etal (2011) Evaluation of the anticancer potential of C.pictus on fibrosarcoma (HT-1080) cell line J.Nat Pharm. 2; 72-76.

Malairaj sathwan etal; (2012) Invitro anti-oxidant and Anti-cancer potential of Bark of Costus pictus D.Don; Asian pacific journal of Tropical Biomedicine. 741-749

Comargo MEM, Wajera C.R, Torres RS, Alderate MEC. (2006) Evaluation of the diuretic effect of the aqueous extract of Costus pictus D.Don in rat. Poc.West Pharmacol Soc. 49; 72-74.

Saju Thomas D.R B Seetha Devi (2013). Phytochemical and invitro anthelmintic studies of hydro alcoholic extract of Costus pictus D.Don. Int J of Pharmacy and Pharmaceutical sciences; Vol 5 Issue 3.

Jeneth Berlin Raj, Rama Krishna kalaivani. (2016) Comparative in-vitro evaluation of anthelmintic property of leaves and rhizome of Costus pictus D.Don against albenzole. National journal of Physiology, Pharmacy and Pharmacology. Vol 6 Issue 5, 438-441.

Jayasri M.A Mathew L Radha A. (2008); A report on the anti-oxidant activity of leaves and rhizome of Costus pictus D.Don. Int.J Integre.Biol 5(1), 20-26.

R Kavitha. (2013). Invitro anti-oxidant studies of Costus pictus leaf. J of Research in Pharmaceutical and Bio medical sciences. Vol 4 (1) Jan- Mar 159-164.

Shiva prakash G. Elizabeth Dilip Thottacherry, Seema rai, Nishchal, Nandhini M, Reshma Kumarachandra, Fahim, Natesh and Pallavi L.C. (2014) Evaluation of anti-oxidant potential of Costus igneus in ethanol induced preoxidative damage in albino rats. Journal of Applied Pharmaceutical Sciences 4 (08): 052-055.

Ramya Urs S.K and Jyothi Bala Chauhan (2015); Phytochemical screening, anti-oxidant activity and anti-oxidant activity of Costus igneus. European journal of Molecular biology and Biochemistry. 2(2) 93-96.

Prejeena V, Suresh S.N and Varsha V. (2017) Phytochemical screening, anti-oxidant analysis and anti-proliferative effect of Costus pictus. D.Don leaf extracts. Int J of Recent Advances in multi-disciplinary Research Vol 4; Issue 03, 2373-2378.

Raju, J., Roa, C.V., (2011); Diosgenin a steroid saponin constituent of yams and Fenugreek; emerging evidence for applications in medicine: In:Rasooli, I (Ed), Bioactive Compounds in Phytomedicine Publisher: In Tech., Rijeka, Croatia.

Yasodha S.Nivedhana Arthi P, Akanksha agarwal (2017), Anti fertility activity of Ethanolic extract of Costus pictus Rhizome in female rats. Scholar’s journal of Applied Medical sciences. 5 (1 A) 62-64.

G.V Srinivasan, K.K Vijayan. P Sharanppa and K.S Jagadeesh (2016); Identification of Chemical compounds in the essential oil from Costus pictus D.Don plant parts antimicrobial studies isolation and quantification of Diosgenin from its roots. J of Chemical and Pharmaceutical Research 8 (7); 594-604.

Veena Sanjay, Gitanjali Deokar and Sanjay Kshirsagar (2016); Pharmacognostic investigation, isolation and evaluation of Diosgenin from Costus pictus.D.Don; International journal of Institutional Pharmacy and Life sciences.

Bakrudeen Ali Ahmed A and Arun kumar R. (2009) In-vitro propagation of monocot Costus pictus D.Don An antidiabetic medicinal plant. Journal of Agricultural Technology. Vol 5(2); 361-369.

K.Punya Pani, J.G Sharma. (2012) Micropropagation and micro-rhizome in Costus pictus.D.Don using in-vitro and ex-vitro nodal segments as explants. Noctulae Scientia Biologicae. 4 (2) 72-78.

Biradar S, Pallavi P and Pandhure (2013). In-vitro Propagation of Costus pictus D.Don. Int J Pharm Bio Sci Vol 4 (4). 918-922.

S.J Wani, I A Kageli, P.S Tamboli, V.S Nirmalkar, S.N Patil, A.K Sidhu. (2014) Optimization of MS media for callus and Suspension culture of Costus pictus. Int J of Scientific and engineering Research Vol 5 issue, 390- 394.

Avinash Jadhav, Vishwanth, Waghwae. (2015) Micropropagation studies in Insulin plant Costus pictus D.Don. Int. J of advanced Research in Computer sciences and Software engineering; Vol 5 Issue 7 607-609.

Sulakshana G, Sabitha Rani A. (2016), Standardization of micropropagation form nodal segments of Costus pictus an antidiabetic plant. Int. J. Current Research Vol: 8 Issue 8 36680-36684.

Azal Khan, Vijay Sharma and R.K Shukla (2017), Establishment of multiplication protocol for insulin plant (Costus pictus.D.Don) by using stem node as explants. International journal of Bio-technology and Research. Vol 7, Issue 1, 11-18.

Ries, S. K.; Wert, V. F.; Sweeley, C. C.; Leavitt, R. A. Triacontanol: a new naturally occurring plant growth regulator. Science 195:1339-1341; 1977

M.P. Morris, B A Roark, Bratolome Cancel (1958) Simple procedure for the routine assay of Dioscorea tubers. Journal Agricultural Food Chemistry 6 (11), pp 856-858.

Trease G.E and Evans W.C (1989). Pharmacognosy; physicians to herbal medicine, 13th edition Bailliere Tindall, London. Pp: 176-180.

Harborne J.B (1973) phytochemical methods. Chapman and Hall, London. P. 113.

Sofawora A (1993) Medicinal plants and traditional medicine in Africa Spectrum books Ltd. Ibadan, Nigerian P-289.

B.A Chindo, B. Adzu, and S.G Karniyus, Saponins(2012): structural Diversity, Properties and Applications, Nova Science Publishers, New York, NY, USA


Refbacks

  • There are currently no refbacks.