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Stability Indicating Spectrophotometric Methods for the Determination of Enrofloxacin in Pharmaceuticals

Nagaraju Rajendra Prasad, Kanakapura Basavaiah, Doora Mahadevaswamy Pramod

Abstract


Enrofloxacin (EFX) is a first fluoroquinolone veterinary antibiotic and used for the treatment of some urinary tract, respiratory tract, and skin infectious diseases in pets and livestock. Three simple, selective and stability indicating UV-spectrophotometric methods have been developed and validated for the assay of EFX in bulk drug and in its dosage forms. The first proposed method (method A) is based on the measurement of the absorbance of EFX in 0.1 N NaOH at 271 nm. The second (method B) and third (method C) methods are based on measurement of absorbance of EFX in 0.1 N HCl and in 0.1 M glacial acetic acid at 277 nm. The calibration graphs are linear over the range 1.0–10.0 μg/mL in all three methods. The correlation coefficient (r) values are evaluated and are found to be 0.9992, 0.9987 and 0.9998 with the apparent molar absorptivity values are 3.17 × 104, 4.42 × 104 and 3.96 × 104 L mol-1cm-1, for method A, method B and method C, respectively. The values of Sandell sensitivity, limits of detection and quantification have also been described. The accuracy and precision of the methods are evaluated based on intra-day and inter-day variations. The stability of the drug is studied by subjecting EFX to an acid and alkaline hydrolysis, oxidative and thermal degradation. The proposed methods are successfully applied to the determination of EFX in tablets and the results obtained are comparable with the published reference method and satisfactory.

Keywords: assay, enrofloxacin, stability indicating, pharmaceuticals, UV-spectrophotometry

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References


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