Open Access Open Access  Restricted Access Subscription or Fee Access

A New Spectrophotometric Method for the Determination of Psilocin and Psilocybin

K. Lavanya, Tulsidas R. Baggi


A rapid new spectrophotometric method is developed for the determination of psilocin and psilocybin using the oxidative coupling reaction with 3-methylbenzthiazolinone-2-hydrazone. The method is precise and accurate. It can be applied to the estimation of psilocin and psilocybin in forensic exhibits.

Keywords: 3-methylbenzthiazolinone-2-hydrazone, psilocin, psilocybin, spectrophotometry

Full Text:



Renolds, J.E.F. (Ed) Martindale – The Extra Pharmacopeia, 28th Edn., The Pharmaceutical Press, London, 1982

O’ Brien, R., Cohen S. The Encyclopedia of Drug Abuse, Green spring Inc., USA, 1984

Farbrick, D.M., Winefordner, J.D., Quantitative measurement of mixtures of hallucinogens by fluorimetry and phosporimetry, Talanta, 20, 1973, 1220

Repke, D.B., Leslie D.T., Mandell D.M., Kish, N.G., GLC – Mass spectral analysis of psilocin and psilocybin, J. Pharm Sci., 66, 1977, 743

Vanhaelen – Fastre, R., Vanhaelen, M., Qualitative and quantitative determination of hallucinogenic components of psilocybe mushrooms by reverse phase HPLC, J. Chromatogr., 312, 1984, 467

Borner, S., Brenneisen, R., Determination of tryptamine derivatives in hallucinogenic mushrooms using HPLC with photodiode array detection, J. Chromatogr., 408, 1987, 402

Beug, M.W., Bigwood, , J, Qualitative analysis of psilocybin and psilocin in psilocybe belocytis (Singer and Smith) by HPLC and TLC, J. Chromatogr., 207, 1981, 379

Lee, R.E., A technique for the rapid isolation and identification of psilocin / psilocybin containing mushrooms, J. Forensic Sci., 30, 1985, 931


  • There are currently no refbacks.