Open Access Open Access  Restricted Access Subscription or Fee Access

Spectrophotometric Determination of Salicylates in Urine using 3-Methylbenzthiazolinone – 2 – hydrazone

N. Geeta, Tulsidas R. Baggi


A sensitive (direct or with column partition chromatography) spectrophotometric method for the determination of salicylates in urine using 3 – methylbenzthiazolinone - 2 – hydrazone in the presence of ceric ammonium sulfate as an oxidizing agent is presented. Beer’s law is obeyed in the concentration range of 0 – 1.6 µg ml -1. The method is accurately reproducible and can be applied in the analysis of salicylates in urine. The method is suitable for toxicological work.

Keywords: 3-methylbenzthiazolinone–2-hydrazine, salicylates, urine, spectrophotometry

Full Text:



Trinder, Rapid determination of salicylate in biological fluids, Biochem, J., 57, 1954, 301.

Stevenson, Rapid UV spectrophotometric determination of salicylates in blood, Anal.Chem., 32,1960, 1522.

You, K,.Polarographic quantification of salicylates in serum by salicylate hydroxylase (salicylate-1 – monooxygenase), Clin. Chim Acta., 149, 281, 1985.

Puetter. J., Determination of salicylic acid and its metabolites: studies in healthy subjects, Arzeneimittal – Forsch, 25, 941, 1975.

Brandson, R. A., Eadie, M. J., Sensitive liquid chromatographic assay for plasma aspirin and salicylate concentration after low dose of asoirin, Therm. Drug. Monit., 7, 216, 1985.

Biber, M. Z, Rhodes, C. J., modification of Trinder’s method for the assay of salicylates in biological fluids, Clinica Acta., 54, 135, 1974.

Chiou, W. L and Onyemelukwe, I., Simple modified colorimetric method for total salicylate assay in urine after salicylate administration, J. Pharm. Sci., 63, 630, 1974.

U. S. Pharmacopoea XXI and National formulary XIV, 1985, U. S. Pharmacopoea Convention Rockville, M. D.

Geeta N and Baggi T. R., A new spectrophotonetric method for determination of free salicylic acid in aspirin and its foprmulations based on oxidative coupling of 3-methylbenzthaiazolinone-2-hydrazone with salicylic acid, Microchem. J. 38, 236, 1988.

Sunshine, I methodology for Analytical Toxicology, C. R. C. Press, 1975.


  • There are currently no refbacks.