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A Simplified High-Performance Liquid Chromatographic Method For Determination of Vitamin D3 in Human Serum

Jaicy Alias, K J Rose Philo


Diabetes is a metabolic disorder caused by defective insulin secretion, insulin action or both. The two major factors for the development of diabetes are pancreatic beta cell dysfunction and insulin resistance. In type 2 diabetes mellitus, production and release of insulin is unobstructed but due to factors like ageing or obesity, the produced insulin cannot compensate for the increased demand. Measurement of the serum level of 25-hydroxyvitamin D3 is the most useful parameter in evaluating vitamin D status. The serum level of vitamin D is a useful parameter in studying short time effects after exposure to ultraviolet light and absorption of the vitamin after oral administration. Method for determination of vitamin D3 is described. The objective of this current study is to analyze the concentration of vitamin D3 in patients with type 2 diabetes mellitus. The samples analysed provide the information that the concentration of vitamin D is low in diabetic patients. This ensure that there is a relationship between the concentration of vitamin D and diabetes.

Keywords: hplc, reversed-phase, type 2 diabetes mellitus

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