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DIRECT DETERMINATION OF AMOXICILLIN SODIUM IN ITS PURE AND DOSAGE FORMS USING ATOMIC ABSORPTION SPECTROMETRY BASED ON ITS SODIUM CONTENT.

Ebrahim Abolmagd, Fathy M Salama, Khalid A Attia, Ahmed A Abouserie, Ahmed El- Olemy

Abstract


Atomic absorbtion spectrometic method has been developed and validated for direct determination of amoxicillin sodium in its pure and pharmaceutical dosage forms based on its sodium content at λ 589 nm. The developed method was found to be time and cost saving and has no tedious or complex procedures. The developed method is very sensetive regarding LOD (0.025 ppm of sodium equivalent to 0.423 μg mL-1), LOQ (0.076 ppm of sodium equivalent to 1.282 μg mL-1) and linearity range (0.2-1 ppm of sodium) equivalent to (3.37-16.84 μg mL-1). The accuracy of the method was further assesed by application of standard addition technique and a very satisfactory results were obtained. The results obtained by the proposed method were compared by statistical measures to reference method and there were no significant difference between the developed method and the reference method regarding the accuracy and precision. The developed method was validated according to ICH guidelines and the results were satisfactory. Keywords: Amoxicillin; Atomic absorption spectrometry.

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References

Coudert M, Vergnaud JM. Atomic absorption spectrometry for direct determination of metals in powders. Anal. Chem. 1970; 42(11): 1303-04.

Al-Ghannam SM. Atomic absorption spectroscopic, conductometric and colorimetric methods for determination of some fluoroquinolone antibacterials using ammonium reineckate. Spectrochimica. Acta Part A, 2008; 69, 1188-94.

Lin TH, Tseng WC, Cheng SY. Direct determination of selenium in human blood plasma and seminal plasma by graphite furnace atomic absorption spectrophotometry and clinical application. Biol. Trace Elem. Res. 1998; 64(1-3): 133-49.

Pereiro R, Sanz-Medel A. Atomic Absorption Spectrometry, 2nd Edition. Momentus press. 2014.

The Merck Index. An Encyclopaedia of Chemicals, Drugs and Biologicals. New Jersey. Published by Merck Research Laboratories, Division of Merck and Co. Inc. Whitehouse station, 14th Ed. 2006, 313.

O'Neil MJ. The Merck index: an encyclopaedia of chemicals, drugs, and biologicals: RSC Publishing; 2013.

B.M. Rahman, K.M. Mahmoud. Batch and Flow Injection Analysis Spectrophotometric Determination of Amoxicillin using N-bromosuccinimide and Indigo Carmine. American Chemical

P. S. Karpova. Quantitative determination of amoxicillin tri hydrate in medical forms using kinetic method. Journal of Chemical and Pharmaceutical Research, 2014, 6(4); 1120-1125.

W.A. AL-Uzri. Spectrophotometric determination of amoxicillin in pharmaceutical preparations through diazotization and coupling reaction. Iraqi Journal of Science, 2012, 53(4).

K. Prakash, P. R. Narayana, K. K. Shanta, N. M. Lakshmi. Spectrophotometric Estimation of Amoxicillin Trihydrate in Bulk and Pharmaceutical Dosage Form. E-Journal of Chemistry, 2008, 5(S2); 1114-1116.

P. De Pourcq, J. Hoebus, E. Roets, J. Hoogmartens, H. Vanderhaeghe. Quantitative determination of amoxicillin and its decomposition products by high-performance liquid chromatography. Journal of chromatography, 1985, 321(2); 441-449.

S. De Baere, De Backer, Patrick. Quantitative determination of amoxicillin in animal feed using liquid chromatography with tandem mass spectrometric detection. Analytica Chimica Acta, 2007, 586(1-2); 319-325.

B. Lugoboni, T. Gazzotti, E. Zironi, A. Barbarossa, G. Pagliuca. Development and validation of a liquid chromatography/tanden mass spectrometry method for quantitative determination of amoxicillin in bovine muscle. Journal of chromatography, 2011, 879(21); 1980-1986.

United States Pharmacopoeia 30 and National formulary 25. Rockville (MD): United State Pharmacopoeia Convention; 2007.

International Conference on Harmonization, ICH Harmonized Tripartite Guideline. Validation of analytical procedure: text andmethodology, Q2 (R1). Geneva: International Conference on Harmonization; 2005.

Armitage P and Berry G. Statistical methods in medical research. 3rd ed. Oxford (UK): Blackwell; 1994.

Parameters Atomic absorption spectrometry Reported method (10)

Na 5 5

Mean 101.69 98.74

Variance 0.481 0.233

%RSD 0.694 0.489

Student’s t-test (2.306)b 1.076 ——

F-value (6.39)b 2.064 ——


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