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Utilization of Mulberry Methanolic Leaf Extractive (MMLE) for Treating Hypercholesterolemia in Rat, Rattus norvegicus (L)
Abstract
Healthy diet is the foundation for a healthier workforce and lower healthcare costs. The diet therapy for regulation of cholesterol is exerting influence to reduce mortality and morbidity. The Methanolic Mulberry Leaf Extractives (MMLE) (at the rate of 100 and 200 mg/kg body weight) and the standard hypolipidemic medicine Questran were administered through the diet to the rats of hypercholesterolemia (maintained on high cholesterol diet). Treatment was five times per week for the period of nine weeks. Analysis of blood plasma and its post-mitochondrial fraction (PMF) was carried out through the bioassay of total cholesterol, triglyceride, lipid peroxidation (LPO) and reduced glutathione. Treating the rats of hypercholesterolemia with MMLE caused the dose dependent reduction in cholesterol level in plasma and it’s PMF. The second dosage of Methanolic Mulberry Leaf Extractives (MMLE) (200 mg/kg) significantly attenuated the cholesterol-induced increase in cholesterol plasma. Similarly, the cholesterol-induced decrease in PMF reduced glutathione levels of hypercholesterolemic rats and were significantly ameliorated in experimental animals treated with Methanolic Mulberry Leaf Extractives (MMLE). The consumption of leaves of mulberry, Morus alba (L) may act as a potent hypocholesterolemic nutrient. It may open a new avenue in the field of regulation of cholesterol through diet.
Keywords: Hypercholesterolemia, Lipid-lowering effect, Morus alba, Cholesterol
References
Durrington, P (August 2003). "Dyslipidaemia". The Lancet. 362 (9385): 717–31. doi:10.1016/S0140-6736(03)14234-1. PMID 12957096.
Biggerstaff KD and Wooten JS (December 2004). "Understanding lipoproteins as transporters of cholesterol and other lipids". Adv Physiol Educ. 28 (1–4): 105–06. doi:10.1152/advan.00048.2003. PMID 15319192.
Carmena R, Duriez P, Fruchart JC (June 2004). "Atherogenic lipoprotein particles in atherosclerosis". Circulation. 109 (23 Suppl 1): III2–7. doi:10.1161/01.CIR.0000131511.50734.44. PMID 15198959.
Kontush A, Chapman MJ (March 2006). "Antiatherogenic small, dense HDL – guardian angel of the arterial wall?". Nat Clin Pract Cardiovasc Med. 3 (3): 144–53. doi:10.1038/ncpcardio0500. PMID 16505860.
de Souza, RJ; Mente, A; Maroleanu, A; Cozma, AI; Ha, V; Kishibe, T; Uleryk, E; Budylowski, P; Schünemann, H; Beyene, J; Anand, SS (11 August 2015). "Intake of saturated and trans unsaturated fatty acids and risk of all cause mortality, cardiovascular disease, and type 2 diabetes: systematic review and meta-analysis of observational studies". BMJ (Clinical Research Ed.). 351: h3978. doi:10.1136/bmj.h3978. PMC 4532752. PMID 26268692.
Ito MK, McGowan MP, Moriarty PM (June 2011). "Management of familial hypercholesterolemias in adult patients: recommendations from the National Lipid Association Expert Panel on Familial Hypercholesterolemia". J Clin Lipidol. 5 (3 Suppl): S38–45. doi:10.1016/j.jacl.2011.04.001. PMID 21600528.
Wald NJ, Law MR. 1995. Serum cholesterol and ischaemic heart disease. Atherosclerosis, 118:1–5.
Krieger M. 1998. The “best” of cholesterols, the “worst” of cholesterols: a tale of two receptors. Proc Natl Acad Sci USA, 95:4077–80.
Schaefer EJ, Lichtenstein AH, Lamon-Fava S, et al. 1995. Lipoproteins, nutrition, aging and atherosclerosis. Am J Clin Nutri, 61:726S–740S.
Adaramoye OA, Nwaneri VO, Anyanwu KC, et al. 2005. Possible antiatherogenic effect of kolaviron (a Garcinia kola seed extract) in hypercholesterolemic rats. Clin Exp Pharmacol Physiol, 32:40–6.
Pritchard KA Jr, Groszek L, Smalley DM, et al. 1995. Native low-density lipoprotein increases endothelial cell nitric oxide synthase generation of superoxide anion. Circ Res, 77:510–18.
Hu SH, Liang ZC, Chia YC, et al. 2006. Antihyperlipidemic and antioxidant effects of extracts from Pleurotus citrinopileatus. J Agric Food Chem, 22:2103–10.
Tomotake H, Yamamoto N, Yanaka N, et al. 2006. High protein buckwheat flour suppresses hypercholesterolemia in rats and gallstone formation in mice by hypercholesterolemic diet and body fat in rats because of its low protein digestibility. Nutr, 22:166–73.
Visavadiya NP, Narasimhacharya AV. 2007. Ameliorative effect of Chlorophytum borivilianum root on lipid metabolism in hyperlipaemic rats. Clin Exp Pharmacol Physiol, 34:244–9.
Miller CA. 2001. Update on statins and other lipid-lowering drugs. Geriatr Nurs, 22:276–77.
Vitthalrao Bhimasha Khyade and Avram Hershko (2018). Use of Aqueous Decoction of Leaves of Mulberry, Morus alba (L) in Hyperglycemia-Induced Oxidative Stress in Brown Rat, Rattus norvegicus (L.). International Journal of Agro-Chemistry Vol 4, No 2 (2018): 01 – 23. eISSN: 2456-7000.www.journalspub.com
Ray Indrajit [1989] : Can Mulberry Provide an Answer to the Scourge of AIDS . Indian Silk, 27 [37-38].
Tewary P.K., G. Subba Rao[1990]: Mulberry By- products and their Utilization .Indian Silk, 29,[43-44].
Vitthalrao Bhimasha Khyade and Avram Hershko* (2018). Mulberry, Morus alba (L): The Global Medicinal Heritage. International Journal of Green Chemistry Vol. 4 No. 2 (2018): 01 -16. www.journalspub.com *: Nobel Prize in Physiology Medicine (2004).
Vitthalrao B. Khyade, James P. Allison (2018). Utilization of the Aqueous Mulberry (Morus alba. L.) Leaf Decoction (AMLD) For Treating the DMBA Induced Hepatotoxicity and Free-Radical Damage in Norwegian Rat, Rattus norvegicus (L). International Journal of Scientific Research in Chemistry (IJSRCH) | IJSRCH | Volume 3 | Issue 5 ISSN: Page 106 – 123. 2456-8457 © 2018 IJSRCH |
Heath RL, Packer L. (1968). Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Archives in Biochemistry and Biophysics 125: 189–198. https://academic.oup.com/jxb/article/52/365/2375/543853
Du Z, Bramlage WJ (1992) Modi®ed thiobarbituric acid assay for measuring lipid oxidation in sugar-rich plant tissue extracts. J Agric Food Chem 40: 1566 -1570. https://www.researchgate.net/publication/225710241_
Merzlyak MN ; Heath RL and Packer L. (1993). Reactive oxygen and oxidative processes in the mem-branes of plant cells. Itogi Nauki i Techniki. Series Fiziologija Rastenij 6, 1-168 (in Russian).http://www.globalsciencebooks.info/Online/GSBOnline/images/2010/PS_4(1)/PS_4(1)44-49o.pdf
Kretzschmar M (1990). The hepatic glutathione system — influences of xenobiotics. Exp Pathol 38:145–164. https://link.springer.com/chapter/10.1007/978-3-642-85036-3_9
Irwin, M , Gougerot-Pacidalo MA, Fay M, Roche Y, Lacombe P & Marquetty C (1975). Immune oxidative injury induced in mice exposed to normobaric oxygen: effects of thiol compounds on the splenic cell sulphydryl content and Con A proliferative response. Journal of Immunology, 135: 2045-2051.
Hashmat Ullah H, Khan MF, Hashmat F. (2011). Determination of glutathione concentration after its interaction with cadmium nitrate tetrahydrate by using Ellman’s modified method. Gomal Uni J Research 2011; 27:18-25.
Muller, F. L., Lustgarten, M. S., Jang, Y., Richardson, A. and Van Remmen, H. (2007). "Trends in oxidative aging theories". Free Radic. Biol. Med. 43 (4): 477-503. doi:10.1016/j.freeradbiomed.2007.03.034. PMID 17640558.
Kwiterovich PO Jr. 1997. The effect of dietary fat, antioxidants, and prooxidants on blood lipids, lipoproteins, and atherosclerosis. J Am Diet Assoc, 97:S31–41.
Yokozawa T, Cho EJ, Sasaki S, et al. 2006. The protective role of Chinese prescription Kangen-karyu extract on diet-induced hypercholesterolemia in rats. Biol Pharm Bull, 29:760–5.
Zhang HW, Zhang YH, Lu MJ, et al. 2007. Comparison of hypertension, dyslipidaemia and hyperglycaemia between buckwheat seed-consuming and non-consuming mongolian-chinese populations in inner mongolia, China. Clin Exp Pharmacol Physiol, 34:838–44.
Fungwe TV, Cagen LM, Cook GA, et al. 1993. Dietary cholesterol stimulated hepatic biosynthesis of triglyceride and reduces oxidation of fatty acids in the rat. J Lipid Res, 34:933–41.
DOI: https://doi.org/10.37628/jcep.v5i2.869
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