Response Surface Methodology to Optimize Deproteinization of Green Crab Shells (Carcinus maenas)
DOI:
https://doi.org/10.37628/jcep.v3i2.397Abstract
The crab shells are used as a feedstock for the extraction of either chitin or chitosan and other bioactive materials such as dyes and proteins. The optimum conditions are required for a process development for an effective separation of chitin. The most important parameters for deproteinization of green crab shells have identified the concentration of NaOH, temperature and time of contact. The optimum conditions for deproteinization of Green crab shells are NaOH concentration (1.25-1.75M), temperature65ͦ C and contact time of 300 mins for effective extraction of protein from the green crab shells. Therefore, Response surface methodology has been applied to the deproteinization of green crab shells for extraction of Chitin or Chitosan. Based on preliminary lab scale deproteinization studies, the minimum and maximum level of the variables are determined for the response surface optimization. Keywords: Deproteinization, Response surface methodology, Box – Behnken design, Green Crab ShellReferences
Chen PY, Structure and mechanical properties of crab exoskeletons, Acta Biomaterialia, 4(3), 2008, 587-596.
New N, Furuike T, and Tamura H, Chapter One - Isolation and Characterization of Chitin and Chitosan from Marine Origin, in Advances in Food and Nutrition Research, K.Se-Kwon, Editor, Academic Press, 2014, 1-15.
Percot A, Viton C, and Domard A, Characterization of Shrimp Shell Deproteinization, Biomacromolecules, 4 (5), 2003, 1380-1385.
Percot A, Viton C, and Domard A, Optimization of Chitin Extraction from Shrimp Shells, Bio macromolecules, 4 (1), 2002, 12-18.
Shanmugam K, Chemical Based Extraction of Chitosan from Green crabs, Halifax, NS: Biomer Innovations Pvt Ltd., 2014.
Shanmugam K, A Modified Process for Deproteinization of Green Grab Shells
(Carcinus maenas) Extraction of Chitin/Chitosan, Journal of Chemical and Pharmaceutical Sciences, Vol 9, 1, 163-165. 2016.
Myer, R. H. (1976). Response surface methodology. USA: Library of Congress
Beth A. Fulton, Elizabeth A. Fairchild, 2013 Nutritional Analysis of Whole Green Crab, Carcinus maenas, for Application as a Fishmeal Replacer in Agrifeeds. Sustainable Agriculture Research, 2(3): 126 -135.
