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Use of Aqueous Decoction of Leaves of Mulberry, Morus alba (L) in Hyperglycemia-Induced Oxidative Stress in Brown Rat, Rattus norvegicus (L.).

Vitthalrao Bhimasha Khyade, Avram Hershko

Abstract


Hyperglycemia is the significant feature of the disease diabetes in human being. The oxidative stress induction is the prime concern with diabetic hyperglycemia. This oxidative stress leads to microvascular and macrovascular complications. Streptozotocin induced diabetic male brown rats ( Rattus norvegicus L.) were divided into five groups, each with 15 individuals. The rats of Group-I served as Normal Healthy group. The Group-II served as streptozotocin induced diabetic rats of solvent treated group. The Group-III served as streptozotocin induced diabetic rats treated with 200 mg/kg decoction of leaves of mulberry, Morus alba (L). The Group-IV served as streptozotocin induced diabetic rats treated with 400 mg/kg decoction of leaves of mulberry, Morus alba (L). The rats in Group- V were treated with glibenclamide (glyburide) (0.5 mg/kg). All the groups were maintained for three weeks. The blood samples were subjected for bioassays of After 3 weeks, blood samples serum glucose, urea and creatinine. For the purpose to confirm the oxidative damage; attempts on lipid peroxidation and histopathology were conducted. Untreated diabetic rats were found with significant increase in serum glucose, urea and creatinine. Significant rise in lipid peroxidation with a glomerular atrophy and necrotic tubular epithelium in the renal tissue was reported in addition. The rise in serum glucose, urea and creatinine was found ameliorated through the decoction of leaves of mulberry, Morus alba (L). The results of the attempt are suggesting that, leaves of mulberry, Morus alba (L) are going to serve as effective nutritional supplement to prevent complications of diabetes. Keywords: Oxidative stress, Kidney, Streptozotocin-induced diabetes, Leaf decoction

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