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Attempt On Utilization of Aqueous Solution of Eurhodin Treated Mulberry Leaves for the Qualitative Cocoons and Silk Filament in silkworm, Bombyx mori (L) Races: Bivoltine Cross Breed [(CSR6 x CSR26) x CSR2 x CSR27)] and multivoltine crossbreed [(PM x CSR

Vitthalrao Bhimasha Khyade, Avram Hershko

Abstract


The vital stain, the Eurhodin, the neutral red dye was used for treating the leaves of mulberry. Hundred grams of fresh mulberry leaves were kept immersed in a liter of aqueous solution of eurhodin powder with hundred milligram per liter (100 ppm) strength for half an hour. Such treated mulberry leaves were drained off completely; fed daily to the fifth instar silkworm larvae of bivoltine Cross Breed [(CSR6 x CSR26) x CSR2 x CSR27)] and multivoltine crossbreed (PM x CSR2) races for first four days after the fourth moult. For each day, four feedings were supplied at the rate 100 grams of mulberry leaves for the group of hundred larvae. Larvae fed with untreated and water treated mulberry leaves were also maintained. Mature larvae were considered for the provision of mountage for spinning the cocoon. The cocoons were harvested on fifth day after the provision of mountage. Treating the mulberry leaves with aqueous solution of eurhodin and feeding fifth instar larvae was found resulted into significant improvement in Tissue Somatic Index (TSI) from 23.855 to 28.499 in multivoltine crossbreed (PM x CSR2) race and from 24.719 to 29.780 in bltivoltine crossbreed [(CSR6 x CSR26) x CSR2 x CSR27)] race. Cocoons spinned by the larvae fed with eurhodin treated mulberry leaves were found effected into fortification in their shell ratio from 19.741 to 24.107 in multivoltine crossbreed (PM x CSR2) race and from 20.975 to 33.393 in bltivoltine crossbreed [(CSR6 x CSR26) x CSR2 x CSR27)] race. The fortification through eurhodin treatment was also reflected in the quality of silk filament, the denier scale. The denier scale was reported to significant improvement from 2.049 to 3.003 in multivoltine crossbreed (PM x CSR2) race and from 3.237 to 4.743 in bltivoltine crossbreed [(CSR6 x CSR26) x CSR2 x CSR27)] race of silkworm, Bombyx mori (L). Through feeding a modified diet mulberry leaves treated with eurhodin, a vital dye, the present attempt is reporting production of red tinged silk filament. This kind of approach may help to establish eco-friendly technology for coloring the natural silk. Key Words: Bombyx mori (L); Eurhodin; Bivoltine; multivoltine; Crossbreed; Denier Scale

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References


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Vitthalrao B. Khyade and Jiwan P. Sarwade ( 2013 ): Utilization of Retinol through the topical application to the fifth instar larvae of silkworm, Bombyx mori (L) (Race: PM x CSR2) for qualitative improvement of the economic parameters. International Journal of Advance Life Sciences Vol. 6 Issue 5 November, 2013.Pages: 532 – 537.www.ijals.comhttp://ijals.com/wp-content/uploads/2014/01/19.-Utilisation-of-Retinol-through-the-topical.pdf

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Vitthalrao B. Khyade and Karel Slama (2015). SCREENING OF ACETONE SOLUTION OF FME AND SELECTED MONOTERPENE COMPOUNDS FOR JUVENILE HORMONE ACTIVITYTHROUGH CHANGES IN PATTERN OF CHITIN DEPOSITION IN THE INTEGUMENT OF FIFTH INSTAR LARVAE OF SILKWORM, Bombyx mori (L) (PM x CSR2). IJBRITISH Vol. 2 Issue 3 (May – June 2015): 68 – 90. ISSN 2349-9419 www.ijbritish.com

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Vitthalrao B. Khyade; Karel Slama; Rajendra D. Pawar and Sanjay V. Deshmukh (2015). Influence of Various Concentrations of Acetone Solution of Retinol on Pattern of Chitin Deposition in the Integument of Fifth Instar Larvae of Silkworm, Bombyx mori (L) (PM X CSR2). Journal of Applicable Chemistry. 2015, 4 (5): 1434 – 1445. www.joac.info

Vitthalrao B. Khyade; Karel Slama; Rajendra D. Pawar and Sanjay V. Deshmukh (2015). Influence of Various Concentrations of Acetone Solution of Retinol on Pattern of Chitin Deposition in the Integument of Fifth Instar Larvae of Silkworm, Bombyx mori (L) (PM X CSR2). Journal of Medicinal Plants Studies. Volume 3 Issue 5 Part C : 124 – 131. http://www.plantsjournal.com/archives/?year=2015&vol=3&issue=5∂=C

Vitthalrao B. Khyade; Poonam M. Patil; Kalyani R. Jaybhay; Rasika G. Gaikwad; Ganga V. Mhamane; Vivekanand V. Khyade; Kavita H. Nimbalkar and Sneha G. Jagtap ( 2007). Effect of digoxin on economic parameters of silk worm, Bombyx mori (L). Journal of Zoological Society of India : Bioinformatics : 23 – 31. (Editors: B. N. Pandey; Sadhana Deshpande; A. K. Triphathi and A. D. Adsool) (Publisher: A P H Publishing Corporation, New Delhi) (ISBN 13: 9788131302200 / ISBN 10: 8131302202).

Vitthalrao B. Khyade; Poonam M. Patil; Kalyani R. Jaybhay; Rasika G. Gaikwad; Ganga V. Mhamane; Vivekanand V. Khyade; Kavita H. Nimbalkar and Sneha G. Jagtap ( 2007). Effect of digoxin on mid gut glucosidase activity in silkworm, Bombyx mori (L). Journal of Zoological Society of India : Bioinformatics : 32 – 48. (Editors: B. N. Pandey; Sadhana Deshpande; A. K. Triphathi and A. D. Adsool) (Publisher: A P H Publishing Corporation, New Delhi) (ISBN 13: 9788131302200 / ISBN 10: 8131302202).

Vitthalrao B. Khyade; Poonam M. Patil; Sharad G. Jagtap; Sunanda V. Khyade and Jeevan P. Sarawade (2010). Effect of Methanolic Extractives of Roots of Achyranthus aspera on Larval Body Wall Chitin in the Fifth Instars of Silkworm, Bombyx mori (L)(Race: PM x CSR2). Advances in Plant Sciences.23(I): 309 313.

Vitthalrao B. Khyade; Sakharam B. Patil ; Sunanda V. Khyade and Ganesh P. Bhawane (2002). Influence of Acetone maceratives of Vitis vinifera on larval parameters of silkworm, Bombyx mori (L). Indian Journal of Comparative Animal Physiology Vol. 21 (1): 14 – 18.

Vitthalrao B. Khyade; Sakharam B. Patil ; Sunanda V. Khyade and Ganesh P. Bhawane (2003). Influence of Acetone maceratives of Vitis vinefera on economic parameters of silkworm Bombyx mori (L).Indian Journal of Comparative Animal Physiology Vol. 21 (1): 28 – 32.

Vitthalrao B. Khyade; Sunanda V. Khyade and Vivekanand V. Khyade (2009). Influence of mealy bug infestation on mulberry leaves on the silkworm, Bombyx mori(L). Eco friendly Insect Pest Management : 325 – 328. (The book edited by Dr. S. Iganacimuthu Director, Entomology Research Institute, Loyola College, Chennai – 600034. ISBN: 81 – 88901 – 37 – 7 . Publisher: Elite Publishing House Pvt. Ltd. New Delhi.

Vitthalrao B. Khyade; Uma S. Ghantaloo and Vandana D. Shinde (2007). Various effects of anti-biotics on selected parameters of silkworm Bombyx mori(L). Journal of Zoological Society of India : Bioinformatics : 11 – 22. (Editors: B. N. Pandey; Sadhana Deshpande; A. K. Triphathi and A. D. Adsool) (Publisher: A P H Publishing Corporation, New Delhi) (ISBN 13: 9788131302200 / ISBN 10: 8131302202).

Vitthalrao B. Khyade; Vivekanand V. Khyade and Rhidim D. Mote (2014). Influence of Acetone extractive of Oroxylum indicum (L) on cocoon characters, silk filament character and electrophoretic patterns of esterase activity of silkworm, Bombyx mori (L) (Race: PM x CSR2). Recent Trends in Zoology(Pages: 12-22). Editor: Dr. R. K. Kasar ; Publisher: Dr. L. S. Matkar (Principal, New Arts, Commerce and Science College, Shevgaon Dist. Ahmednagar – 414502 (M.S.) India. ISBN: 978-93-84916-68-8.

Vitthalrao B. Khyade; Vrushali D. Shinde and Shraddha S. Maske (2016). Influence of the diterpenoids (Retinol and Phytol) (Race: PM x CSR2) on the cocoon and silk parameters in silkworm, Bombyx mori (L) (Race: PM x CSR2). World Scientific news 42 (2016): 1-12. www.worldscientificnews.com .

Vitthalrao B.Khyade ; Vivekanand V. Khyade and Randy Wayne Schekman (2015). Utilization of the topical application of Limonene to the fifth instar larvae of the silkworm, Bombyx mori (L) (Race: PM X CSR2) for the parameters of Larvae, Cocoon and Silk filament. International Journal of Bioassay 4 (02): 3632 – 3635.ISSN: 2278-778X www.ijbio.com

Vitthalrao Khyade, Edvard Moser and May – Britt Moser (2015). INFLUENCE OF AQUEOUS MACERATIVES OF SEED POWDER OF SYZIGIUM CUMINI (L) ON THE MID GUT ENZYME ACTIVITY IN THE FIFTH INSTAR LARVAE OF SILK WORM, BOMBYX MORI (L) (Race: PM x CSR2). World Journal of Pharmaceutical Research Volume 4, Issue 6:997 – 1008. (ISSN 2277– 7105).www.wjpr.net.

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Vitthalrao Bhimasha Khyade (2018). Use of Aqueous Solution of Eurhodin Treated Mulberry Leaves for the Qualitative Cocoons and Silk Filament in silkworm, Bombyx mori (L) Races: Bivoltine Cross Breed [(CSR6 x CSR26) x CSR2 x CSR27)] and multivoltine crossbreed [(PM x CSR2)]. International Journal of Scientific Research in Chemistry (IJSRCH) | ISSN: 2456-8457 © 2018 IJSRCH | Volume 3 | Issue 5: 19-34. http://ijsrch.com/archive.php?v=3&i=7&pyear=2018

Sampada Ravindra Kakade, Pallavi Ramesh Lonkar, Vitthalrao Bhimasha Khyade (2018). Influence of Aqueous Solution of Agaricus bisporus (L) Treated Mulberry Leaves on the Quality of cocoons and silk filament in silkworm, Bombyx mori (L). International Journal of Scientific Research in Chemistry (IJSRCH) | ISSN: 2456-8457 © 2018 IJSRCH | Volume 3 | Issue 5: 39-51.

http://ijsrch.com/archive.php?v=3&i=7&pyear=2018


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