Purification of Plasmodium Recombinant Protein

Abstract

Malaria is a protozoan disease, caused by parasite plasmodium. There are several species of plasmodium like as P. vivax, P. Ovule, P. Falciparum. It is globally spreaded disease. In 2017, there were an estimated 219 million cases of malaria in 87 countries & deaths stood at 435 000 (WHO.Which protein we purified, we only knew the sequence. So if we could know the function of the protein then we can design a new drug against malaria or block the protein function. For all these possibilities we have to know about protein function by purify and analyse protein. This purification method gave the easy way to purify the recombinant protein. The Ni-NTA (Nickel Ni trilo acetic acid) column purify the protein by the help of charge, first the nickel has to be attached with beads (agarose) and the protein should be poly-histidine tag. The his tag protein bind with nickel and remain in the last, validation and analysis of desired protein by SDS PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis) & western blotting, after SDS the gel was stained by coomassie blue dye and destain by glacial acetic acid & methanol, result observed positive, the band was presented, analysis of protein obtained by Western blotting and the result was negative. The band was not appeared