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Synthesis, Characterization and Optimization of Essential Oil from Pumpkin Seed

Meseret Ethiopia Guye, Mintesinot Dessalegn Dabaro

Abstract


The main aim of this research work was extraction, characterization and optimization of essential oil from pumpkin seed, cucurbita pepo variety using ethanol as a solvent. The extraction was carried out by using a Soxhlet, leaching method and oil was separated from solvents by using rotary evaporator. Pumpkin seed was analyzed for proximate analysis and found that moisture content of 5.21 , crude proteins content of 22.50.31%, crude fat content of 50.50.112%, crude fiber content of 70.09% ash content of 4.50.19%, and carbohydrate content of 10.370.079%. A full factorial experimental randomized design with three factors two levels with triplicate six center points with a total of 30 experimental runs was carried out to optimize the operational process factors on the yield of essential oil. The factors and their operating ranges were: extraction time from 2 to 6 hours, particle size from 0.25 mm to 2mm, and solvent to pumpkin seed meal ratio of 0.05mg/ml to 0.1 mg/ml under constant temperature of 80. Physic-chemical properties of extracted essential oil analyzed and found that specific gravity of 0.91178, pH of 5.160.1kinematic viscosity 35, density 911.78, moisture content 0.07%, volatile contents 1.468, refractive index 0.620.61, free fatty acid 97.5I2g/100g, saponification value of 1.230.23mg/KOH/g oil and 189.80.31mgKOH/g oil, which is in close agreement with the literature values. The FT-IR analysis shown that the presence of functional group of carbohydrate, carbonyl, alkene, aromatics, alkane, aliphatic amine, carboxylic acid, alcohol and methyl ester which is closest to essential oil composition in the literature. Gas-Chromatography-Mass Spectroscopy analysis shown that the presence of five major free fatty acids: linoleic acid of 57.5%, oleic acid of 20.2%, palmitic acid 19.1%, stearic acid 2.97%, and miystiric acid 0.23%. The pumpkin seed essential oil also showed better antimicrobial activity against both S. aureus and E. coli with maximum zone of inhibition 15.50.23 mm and 13.50.51 mm, respectively. Therefore, pumpkin seed essential oil, it is feasible to be used as antimicrobial and other applications


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Meru G., Fu Y., Leyva D.(November, 2017). Health Benefits of Pumpkin Seed and Nutrition Profile of 35 Pumpkin Accessions[online]. Available from https://edis.ifas.ufl.edu/

publication/HS1312#:~:text=Pumpkin%20seed%20is%20a%20nutritious,the%20oil%20(Bavec%20et%20al.

Properzi A., Angelini P., Venanzoni R. Some Biological Activities of Essential Oils. Med Aromat Plants. 2013; 2(5).

Sartoratto A., Machado A. L. M., Delarmelina C., et.al. Composition and antimicrobial activity of essential oils from aromatic plants used in brazil. Brazilian Journal of Microbiology. 2004; 35(4):275-280.

Martha, R., Gutierrez, P. Review of Cucurbita pepo (Pumpkin) its Phytochemistry and Pharmacology. Medicinal chemistry. 2016; 6(1): 12–21.

Balami, S. (2007). Addis Ababa University.

Stevenson D. G., Eller F. J., Wang L., et.al. Oil and Tocopherol Content and Composition of Pumpkin seed oil in 12 cultivars. J Agric Food Chem. 2007; 55(10): 4005-4013.

Malley, B. O. Cucurbitaceae. America: American Press; 2008.

Marie A. Niangoran N., Digbeu Y. D., Binaté S., et.al. Fatty Acid Composition and Physicochemical Properties of Four Varieties of Citrullus lanatus Seed Oils Cultivated in Côte d ’ Ivoire. Biotechnology Journal International. 2015; 5(3): 140–147.

Jafari M., Amir S., Goli H., et.al. The chemical composition of the seeds of Iranian pumpkin cultivars and physicochemical characteristics of the oil extract. European Journal of Lipid Science & Technology. 2012;114(2): 161–167.

Sito S., Voc N., Barc J. Differences in water release rate of hulled and hull-less mit und ohne Schale. Croatian scientific bibliography. 2005; 56(3): 153–159.

Abdel-Rahman, M. K. Effect of Pumpkin Seed (Cucurbita pepo L.) Diets on Benign Prostatic Hyperplasia (BPH): Chemical and Morphometric Evaluation in Rats. World Journal of Chemistry. 2006; 1(1): 33–40.

Bavec, F., Mlakar, S. G., Rozman, et.al. (2007). Oil Pumpkins: Niche for Organic Producers [online]. Available from https://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.509.8834&

rep=rep1&type=pdf.

Bwade K. E., Aliyu B., Kwaji A. M., et.al. I Physicochemical Properties of Pumpkin Seed Oil Relevant to Bio-diesel Production and other Industrial Applications. nternational Journal of Engineering, Business and Enterprise Applications (IJEBEA). 2020; 4:72–78.

Eze, S. O. O. Physico-chemical properties of oil from some selected underutilized oil seeds available for biodiesel preparation. African Journal of Biotechnology. 2012; 11(42), 10003–10007.

Adeel Asma Sohail Rabia, Masud Tariq. Characterization and antibacterial study of pumpkin seed oil (cucurbita pepo). Life Sciences Leaflets. 2014; 49: 53–64.

Orsavova J., Misurcova L., Ambrozova J. V.,et.al. Fatty Acids Composition of Vegetable Oils and Its Contribution to Dietary Energy Intake and Dependence of Cardiovascular Mortality on Dietary Intake of Fatty Acids. Int. J. Mol. Sci. 2015; 16(6): 12871-12890.

Popa V.-M., Hădărugă N. G., Hădărugă D. I., et.al. Fatty acids composition of some vegetable oils obtained in the west area of Romania. Journal of Agroalimentary Processes and Technologies. 2010; 16(3): 394–398.

Petkova Z. Y., Antova G. A. Changes in the composition of pumpkin seeds (Cucurbita moschata) during development and maturation. Grasas Aceites. 2014; 66(1): 1–9.

Hamed S. Y., Hassan N. M. El, Hassan A. B., et.al. Nutritional Evaluation and Physiochemical Properties of Processed Pumpkin (Telfairia occidentalis Hook) Seed Flour. Pakistan Journal of Notrition. 2008; 7(2): 330–334.

A.A.C.C. Approved Methods of the American Association of Cereal chemists. 10th Ed. St. Paul, MN, USA: AACC; 2000.

A.O.A.C. (2000). Association of official analytical chemists. Official method 920.212, Saponification on matter of oil and fat, 17thedition.

A.O.A.C. (2000). Association of official analytical chemists. Official method 920.212, specific gravity (apparent) of oil, density bottle method. 17thedition.

Elinge C. M., Muhammad A., Atiku F. A., et.al. Proximate, Mineral and Anti-nutrient Composition of Pumpkin (Cucurbitapepo L) Seeds Extract. International Journal of Plant Research.2012; 2(5), 146–150.

Horwitz William. Official Methods of Analysis of Association of Official Analytical Chemists. 17th ed. Rockville MD: AOAC International; 2003.

A.O.A.C. (2000). Association of official analytical chemists. Official method 993.20, Iodine value of oil and fat.

Nurrulhidayah A. F., Che Man Y. B., Al-Kahtani H. A., et.al. Application of FTIR spectroscopy coupled with chemometrics for authentication of Nigella sativa seed oil. Journal of Spectroscopy. 2011; 25(5): 243–250.

International. In: Horwitz, W. (Ed.), 17thed. AOAC Press, Arlington, VA, USA.

Montgomery, D. C. Applied Statistics and Probability for Engineers. 3rd ed. U.S: Wiley; 2002.

Moo-huchin V., Estrada-mota I., Estrada-león R., et.al. Chemical composition of crude oil from the seeds of pumpkin (Cucurbita spp .) and mamey sapota (Pouteria sapota Jacq.) grown in Yucatan, Mexico, CyTA-Journal of Food. 2013;11(4): 324-327.

Armando, Rahma. Evaluation of the yield and the antimicrobial activity ofthe essential oils from: eucalyptus globulus, cymbopogon citratus and rosmarinus officinalis in mbarara district (Uganda). Rev. Colombiana cienc. Anim.2009; 1(2): 240-249.

Del-vechio-vieira, G., Sousa, O. V, Yamamoto, C. H.,et.al. Chemical Composition and Antimicrobial Activity of the Essential Oils of Ageratum fastigiatum ( Asteraceae ).Records of Natural Products. 2009; 3(1): 52-57.

Milani, E., Razavi, M. S. A., Koocheki, A., Nikzadeh, V., Vahedi, N., & Moeinfard, M. (2007). Riz Chiny Gushty, (1993), 157–168.

Gohari Ardabili A., Farhoosh R., Haddad Khodaparast M. H. Chemical composition and physicochemical properties of pumpkin seeds (Cucurbita pepo subsp. pepo Var. styriaka) grown in Iran. Journal of Agricultural Science and Technology. 2011; 13: 1053–1063.

Kwiri R., Winini C., Musengi A., et.al. Proximate composition of pumpkin gourd ( Cucurbita pepo) seeds from Zimbabwe. International Journal of Nutrition and Food Sciences. 2014; 3(4): 279–283.

Mathangi S. A study on extraction of oil from Pumpkin seed using sun drying and hot air oven drying. International Journal of Food Science and Nutrition. 2018; 3(1): 34–36.

Widy-tyszkiewicz E., Widy-tyszkiewicz E. (2013). Assessment report on Cucurbita pepo L., semen, 44 [online]. Available from https://www.ema.europa.eu/en/documents/herbal-report/final-assessment-report-cucurbita-pepo-l-semen_en.pdf.

Liauw M. Y., Natan F. A., Widiyanti P., et.al. Extraction of neem oil (a zadirachta indica a. Juss) using n-hexane and ethanol : studies of oil quality, kinetic and thermodynamic. ARPN Journal of Engineering and Applied Sciences. 2008; 3(3): 49–54.

Nwofia G. E., Victoria N. N., Blessing K. N. Nutritional Variation in Fruits and Seeds of Pumpkins (Cucurbita Spp) Accessions from Nigeria. Pakistan Journal of Nutrition. 2012; 11(10): 946-956.

Stevenson D. G., Eller F. J., Wang L., et.al. Oil and Tocopherol Content and Composition of Pumpkin seed oil in 12 cultivars. J Agric Food Chem. 2007; 55(10): 4005-4013.

Rodríguez-miranda J., Hernández-santos B., Herman-lara E., et.al. Effect of some variables on oil extraction yield from Mexican pumpkin seeds. CyTA–Journal of Food. 2014; 12(1): 9–15.

A.O.A.C.(2000). Association of official analytical chemists. Official method 960.19, Acid value of oil and fat, 14thedition.

A.O.C.S. (1998). Official methods and recommended Practices of AOCS. American oil

Napier, T. (2009). Pumpkin production, (November).

Srbinoska M., Hrabovski N., Rafajlovska V., et.al. Characterization of the seed and seed extracts of the pumpkins Cucurbita maxima D. and Cucurbita pepo L. from Macedonia. Macedonian Journal of Chemistry and Chemical Engineering. 2012; 31(1): 65–78.

Tsaknis J., Lalas S., Lazos E. S. Characterization of crude and purified pumplcin seed oil. Grasas Y Aceites Fase. 1997; 48(5): 267–272.

Bikash, B., Choudhury, N. D., Bora, D. K. Physicochemical Assessment of Pumpkin (Cucurbita pepo L.) Seed Oil as a Viable Feedstock for Biodiesel Production Oil extraction.Singapore: Springer Singapore; 2018.


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