Bioethanol Production from Corncob Cellulosic Biomass: RSM and ANN based Optimization for Dilute Acid Pretreatment

Amen Aniley Amentie

Abstract


To increase the yield of fermentable sugars, the complexity of lignocellulosic biomass requires a pretreatment method. Dilute acid hydrolysis was used to efficiently pretreatment corn cobs. The aim of this study was to characterize corncob and investigate the effect of process parameters on total reducing sugar (TRS) yield during dilute acid hydrolysis pretreatment of corncob biomass. The morphology and microstructure of the samples were examined using an X-ray diffraction machine, a scanning electron microscope (SEM), and Fourier transforms infrared (FT-IR) spectroscopy (XRD). Corn cobs were immersed in varying concentrations of sulfuric acid at varying temperatures and hydrolysis times. To obtain the reducing sugar for analysis, the corn cobs were hydrolyzed. The individual and interdependent effects of these hydrolysis factors were investigated using a central composite experimental design (CCD). Three factors were examined in acid pretreatment: temperature, time, and acid concentration. At the optimized condition (2.25% (v/v) H2SO4, 126.5°C, and 73 min), maximum TRS obtained was 19.9 g/L.

References


A. I. Anukam, B. P. Goso, O. O. Okoh, and S. N. Mamphweli. (2017). Studies on Characterization of Corn Cob for Application in a Gasification Process for Energy Production. J. Chem.

A. I. Anukam, S. N. Mamphweli, P. Reddy, and O. O. Okoh. (2016). Characterization and the effect of lignocellulosic biomass value addition on gasification efficiency. Energy Explor. Exploit.

A. Singh and N. R. Bishnoi. (2012). Optimization of ethanol production from microwave alkali

pretreated rice straw using statistical experimental designs by Saccharomyces cerevisiae. Ind. Crops Prod.

A. Sluiter, R. Ruiz, C. Scarlata, J. Sluiter and D. Templeton. (2008). Determination of Extractives in Biomass: Laboratory Analytical Procedure (LAP). NREL/TP-510-42619.

al., A. A. (2015). Characterization of a multi-metal binding biosorbent: Chemical modification and desorption studies. Bioresour. Technol.

al., S. e. (2012). Determination of structural carbohydrates and lignin in Biomass. NREL/TP-510– 42618 analytical procedure.

al., T. E. (1998). Chemical Analysis and Testing Laboratory Analytical Procedures. NREL-

Protocols.

B. D. Cullity and S. R. Stock. (2001). Elements of X-ray diffraction, 3rd edition. Prentice Hall.

B. Hames, F. Posey-eddy, C. Roth, R. Ruiz, and D. Templeton. (2002). Procedure .

B. Hames, R. Ruiz, C. Scarlata, A. Sluiter, J. Sluiter, and D. Templeton. (2008). Preparation of Samples for Compositional Analysis Laboratory.

Badal C. Saha, Nancy N. Nichols, Michael A. Cotta. (2005). Ethanol production from wheat straw by recombinant Escherichia coli strain FBR5 at high solid loading. Bioresource Technology.

Dawson L, Boopathy R . (2008). Cellulosic ethanol production from sugarcane bagasse without enzymatic saccharification. BioResources, 452–460.

Fialho, J. (2015). Development of selective fractionation methods for the integrated upgrade of corn cobs in the biorefinery framework.

G. L. Miller. (2002). Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar.Anal. Chem.

Gebrejewergis, A. T. (2019). Addis Ababa Institute of Technology School of Chemical & Bio-

Engineering. Academia.

J. Coates. (2006). Interpretation of Infrared Spectra, A Practical Approach. Encyclopedia of

Analytical Chemistry.

M. Idrees, A. Adnan, S. A. Bokhari, and F. A. Qureshi,. (2014). Production of fermentable sugars by combined chemo-enzymatic hydrolysis of cellulosic material for bioethanol production. Brazilian J. Chem. Eng.

N. W. Bower, Skoog, D. A., and Leary, J. J. (1992). Principles of Instrumental Analysis. 4th edition. J. Chem. Educ. Commented [s5]: Please check: All references are not

citedInternational Journal of Chemical Engineering and Processing Volume 9, Issue 2

ISSN: 2455-5576 © JournalsPub 2023. All Rights Reserved 34

P. Kahar, K. Taku, and S. Tanaka,. (2010). Enzymatic digestion of corncobs pretreated with low strength of sulfuric acid for bioethanol production. J. Biosci. Bioeng.

Rezaei B, Havannavar, and Geeta SG. (2008). Pre-treatment of Agroresidues for Release of

Maximum Reducing Sugar. Karnataka J. Agric. Sci., 771–772.

S. Chongkhong and C. Tongurai,. (2018). Optimization of glucose production from corncob by microwave-assisted alkali pretreatment and acid hydrolysis. Songklanakarin J. Sci. Technol.,

–562.

S. Kumar, Y. S. Negi, and J. S. Upadhyaya. (2010). Studies on characterization of corn cob based nanoparticles. Adv. Mater. Lett.

S. Naik, V. V. Goud, P. K. Rout, K. Jacobson, and A. K. Dalai,. (2010). Characterization of

Canadian biomass for alternative renewable biofuel. Renew. Energy.

S. Sasmal, V. V. Goud, and K. Mohanty. (2012). Characterization of biomasses available in the region of North-East India for production of biofuels. Biomass and Bioenergy.

Takagi SN, Abe S, Suzuki S, Emert GH, Yata N . (1977). In Bioconversion of Cellulosic Substances into Energy, Chemicals and Microbial Protein. Indian Institute of Tsechnology, 551–571.

Teng-Chieh Hsu, Gia-Luen Guo, Wen-Hua Chen, Wen-Song Hwang. (2010). Effect of dilute acid pretreatment of rice straw on structural properties and enzymatic hydrolysis. Elsevier scienece direct.

Y. Cao and H. Tan. (2005). Study on crystal structures of enzyme-hydrolyzed cellulosic materials by X-ray diffraction. Enzyme Microb. Technol.

Ying Y, Shivappa RS, Burns JC, Cheng JJ. (2009). Dilute Acid Pretreatment of Oven-dried

Switchgrass Germplasms for Bioethanol Production. Ener. Fuels.


Refbacks

  • There are currently no refbacks.