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Bioremediation of Polluted Drainage with Petroleum Hydrocarbon

Ukpaka C. P., Nwosi-Anele A. S, Chie-Amadi G. O., Chuku B.

Abstract


This research was aimed at examining the petroleum product discharge in drainage within Port Harcourt City, samples were collected and mixed. Microbial analysis and physicochemical properties of the sample were determined. The sample was subjected into bioremediation after culturing of the microorganisms isolated, identified and characterized. The two microorganisms used are pseudomonas aeruginosa and aspergillus niger. The results obtained revealed high concentration for heavy crude oil than mixed crude oil; also it is observed that the two species of the microbes used are capable of remediating a pollutant environment. The specific growth rate of substrate and microbes, maximum specific growth rate of substrate and microbes as well as the equilibrium or dissociation constant was computed as demonstrated in this research. The challenges uncounted in the remediation of polluted drainage influence the effectiveness of the remediation programme. Indeed, constant discharge of waste into the drain as well as constant remediation is required, but the continuous discharged inhibit the effectiveness of the microorganism inoculated into the system because constant change in the substrate concentration. The activation of the microorganism will speed up the action of degradation, substrate utilization and the required period to achieve perfect clean-up. The kinekic model demonstrates the various mechanisms which the coefficients values can be evaluated and the obtained values usefulness in predicting the degradation period when the active site of the capable organisms are not inhibited

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References


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